The Portuguese attacker, who has netted 12 goals this league campaign, was replaced at half-time of Liverpool’s 2-0 win away to Inter Milan in the UEFA Champions League in midweek.
Reds boss Jurgen Klopp admitted after the game that he had a “swollen ankle” but would wait on further examination, with the club since confirming he’ll miss Saturday’s game.
“No, he will not be available but the extent is still not clear,” Klopp told reporters on Friday.
“We need further assessment. It’s something with some ligaments in and around the ankle, but not the ligament, some others.
“And so, pretty much everything is possible in the moment: that it will go really quick and the other way around unfortunately as well. So we have to wait.
“Everybody saw the picture with him in the boot, it’s a normal procedure; even when you just feel something they put you in that boot. For the weekend, for sure not.”
Jota’s absence is a blow for the Reds, but they have been boosted by Mohamed Salah and Sadio Mane’s returns from the Africa Cup of Nations where they face off in the final.
The Reds have a busy period of fixtures coming up, with a home clash with Leeds United in midweek, while the League Cup final against Chelsea is approaching on Sunday week too.