Record-keeping began in 1991 and he is now the all-time leader in aces served.
It’s actually really cool,” said 37-year-old Isner, ahead of Friday’s match.
“It’s something I’m really proud of. I will be the all-time leader. I’ll keep playing, keep adding to my total… I don’t know if (the record) will get broken. I could be up there for a long time.
Earlier in Wimbledon, Isner had dumped British tennis player Andy Murray 6-4, 7-6, 6-7, 6-4. It ended Murrays campaign and hopes of making it further in the tournament.
He celebrated his win over Murray by pumping his fist.
The age Im at now, I need to relish these moments. This was one of the biggest wins of my career, just given the atmosphere, [which] was fantastic, said Isner. To play as well as I did against one of our greatest players ever, Andy Murray, was a huge accomplishment for me.
In addition to this recent record, Isner who is six feet ten inches tall also holds the record for being part of the longest match in the history of tennis. This was in 2010 against French player Nicholas Mahut. It lasted 11 hours and five minutes and is commemorated outside Court 18 of Wimbledon.
Of his newest achievement, John McEnroe said, Give him another plaque.