It represents another European failure for the Old Lady of Turin, so dominant domestically, but who have only won the elite European competition twice, with the most recent of those triumphs coming as far back as 1996.
On the night they can count themselves a little unfortunate. Trailing by a single goal from the first leg in France in March before the lockdown, they suffered an early blow when Lyon were awarded a very soft penalty, which replays later suggested should never have been given. However, Memphis Depay calmly kept his nerve with a Panenka, meaning that Juventus needed to score three to progress.
As ever they looked to their talisman, Cristiano Ronaldo for inspiration, and he did not disappoint, equalising with a penalty of his own, and then giving his side the lead on the night with a fine individual goal. That took his tally in the Champions League to 130 goals, and also broke a Juventus record that had stood for 94 years, beating the tally of Ferenc Hirzer of goals in a single season for Juventus.
However, he could not find a third, and Juventus were left to pick up the pieces after another failed European campaign.
And that exit could well cost Sarri his job. The former Napoli boss was appointed at the start of the season after a single year with Chelsea, where he had won the Europa League and achieved a top four finish, but failed to endear himself to the clubs fans with his style of play.
He duly won Serie A, but that is a de minimis requirement for a Juventus manager, and he hardly did it in style. His side won only two of their last eight games, and stumbled over the finishing line, finishing just one point ahead of Inter Milan.
And they also lost the Italian Cup final to his old team Napoli on penalties in the first match after lockdown.
More was expected of the 60-year-old and suitors to replace him are already being lined-up.